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Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

DOI :

10.3791/58297-v

12:06 min

January 11th, 2019

January 11th, 2019

11,618 Views

1Center for Cancer Research, Massachusetts General Hospital, 2Department of Molecular Genetics, Weizmann Institute of Science

Primordial germ cells (PGCs) are common precursors of both sperm and eggs. Human embryonic PGCs are specified from pluripotent epiblast cells through interactions of cytokines. Here, we describe a 13-day protocol of inducing human cells transcriptomally resembling PGCs at the surface of embryoid bodies from primed-pluripotency induced pluripotent stem cells.

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