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Discovery of Metastatic Regulators using a Rapid and Quantitative Intravital Chick Chorioallantoic Membrane Model

DOI :

10.3791/62077-v

February 3rd, 2021

February 3rd, 2021

2,368 Views

1Department of Oncology, University of Alberta, Edmonton, Alberta, Canada, 2Department of Cell Biology & Anatomy, Cumming School of Medicine, University of Calgary

This is an effective method to screen for suppressors or drivers of cancer metastasis. Cells, transduced with an expression library, are injected into the chicken chorioallantoic membrane vasculature to form metastatic colonies. Colonies having decreased or increased invasiveness are excised, expanded, reinjected to confirm their phenotype, and finally, analyzed using high throughput sequencing.

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