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Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons

DOI :

10.3791/58421-v

10:50 min

November 2nd, 2018

November 2nd, 2018

49,658 Views

1UNC Neuroscience Center, 2UNC/NC State Joint Department of Biomedical Engineering, UNC, 3Xona Microfluidics, LLC

This protocol describes the use of plastic chips to culture and compartmentalize primary murine neurons. These chips are preassembled, user-friendly, and compatible with high-resolution, live, and fluorescence imaging. This protocol describes how to plate rat hippocampal neurons within these chips and perform fluidic isolation, axotomy and immunostaining.

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Pre assembled Plastic Microfluidic Chips

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