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Compartmentalization of Human Stem Cell-Derived Neurons within Pre-Assembled Plastic Microfluidic Chips

DOI :

10.3791/59250-v

6:46 min

May 3rd, 2019

May 3rd, 2019

65,190 Views

1UNC Neuroscience Center, 2UNC/NC State Joint Department of Biomedical Engineering, 3Xona Microfluidics, LLC

This protocol demonstrates the use of compartmentalized microfluidic chips, injection molded in a cyclic olefin copolymer to cultured neurons differentiated from human stem cells. These chips are preassembled and easier-to-use than traditional compartmentalized poly(dimethylsiloxane) devices. Multiple common experimental paradigms are described here, including viral labeling, fluidic isolation, axotomy, and immunostaining.

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Compartmentalization

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