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Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers

DOI :

10.3791/58545-v

June 4th, 2019

June 4th, 2019

7,236 Views

1Biological Sciences Department Hunter College, City University of New York, 2Program in Molecular, Cellular, and Developmental Biology, Graduate Center, City University of New York

Here, we present a protocol for the visualization, detection, analysis and tracking of endogenous mRNA trafficking in live Drosophila melanogaster egg chamber using molecular beacons, spinning disc confocal microscopy, and open-source analysis software.

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