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How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells

DOI :

10.3791/58588-v

January 7th, 2019

January 7th, 2019

6,338 Views

1Gynecologic Oncology Division, Stanford University School of Medicine

Here, we present a protocol that involves genetically coupled spectrally distinct photoactivatable and fluorescent proteins. These fluorescent protein chimeras permit quantification of the PA-FP fraction that is photoactivated to be fluorescent, i.e., the photoactivation efficiency. The protocol reveals that different modes of photoactivation yield different photoactivation efficiencies.

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