JoVE Logo
Faculty Resource Center

Sign In

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect

DOI :

10.3791/58594-v

6:42 min

March 19th, 2019

March 19th, 2019

5,330 Views

1European Synchrotron Radiation Facility, Structural Biology Group, 2European Molecular Biology Laboratory, 3Univ. Grenoble Alpes, CNRS, CEA, IBS (Institut de Biologie Structurale)

We present the use of the MeshAndCollect protocol to obtain a complete diffraction data set, for use in subsequent structure determination, composed of partial diffraction data sets collected from many small crystals of the fluorescent protein Cerulean.

Tags

MeshAndCollect

-- Views

Related Videos

article

The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination

article

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

article

Analysis of Fluorescent-Stained Lipid Droplets with 3D Reconstruction for Hepatic Steatosis Assessment

article

Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus

article

Author Spotlight: Peptidome Extraction from Small Extracellular Vesicles Isolated from Bone Marrow-Derived Macrophages

article

Author Spotlight: A Bicelle Crystallization Setup for ABC Transporter Membrane Proteins to Advance Drug Development

article

Author Spotlight: An Alternative Approach to Protein Quantification by Bradford Assay Using a Smartphone

article

Author Spotlight: Expression and Purification of Human Solute Carrier Transporters Using Codon-Optimized Genes

article

Author Spotlight: Enhancing CryoEM Sample Preparation Using Graphene Monolayer on Microscopy Grids

article

Reductive Methylation and Pre-Crystallization Treatment of Purified Heterodimeric ABCG5/G8 Protein

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved