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Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast

DOI :

10.3791/58748-v

November 28th, 2018

November 28th, 2018

6,647 Views

1Department of Anatomy and Cell Biology, University of Western Ontario, 2Department of Pathology and Laboratory Medicine, University of Western Ontario

This article describes protocols to assess the effect of fluorescent proteins on the aggregation and toxicity of misfolded polyglutamine expansion for the rapid evaluation of a newly uncharacterized fluorescent protein in the context of fluorescent reporters.

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Fluorescent Proteins

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