JoVE Logo
Faculty Resource Center

Sign In

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

DOI :

10.3791/52610-v

11:19 min

May 4th, 2015

May 4th, 2015

10,818 Views

1Department of Microbiology, University of Washington, 2Departments of Medicine and Laboratory Medicine, University of Washington, 3U.S Military HIV Research Program, Walter Reed Army Institute of Research, 4Henry M. Jackson Foundation

Growth competition between nearly isogenic viruses provides a sensitive measurement for determining relative replication fitness. The protocols described here include the construction of recombinant HIV-1 clones, virus propagation and growth competition and analysis methods optimized to yield sensitive and consistent results.

Tags

Pairwise Growth Competition Assay

-- Views

Related Videos

article

Determining the Reactivity and Titre of Serum using a Haemagglutination Assay

article

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses

article

Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System

article

Determining Soil-transmitted Helminth Infection Status and Physical Fitness of School-aged Children

article

A Visual Assay to Monitor T6SS-mediated Bacterial Competition

article

Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro

article

A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses

article

Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity

article

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

article

Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved