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Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)

DOI :

10.3791/4002-v

10:16 min

May 5th, 2012

May 5th, 2012

37,712 Views

1Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish General Hospital, 2Department of Microbiology and Immunology, McGill University , 3Department of Medicine, Division of Experimental Medicine, McGill University

A fluorescence in situ hybridization (FISH) method was developed to visually detect viral genomic RNA using fluorescence microscopy. A probe is made with specificity to the viral RNA that can then be identified using a combination of hybridization and immunofluorescence techniques. This technique offers the advantage of identifying the localization of the viral RNA or DNA at steady-state, providing information on the control of intracellular virus trafficking events.

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Viral RNA

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