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Cerebellar Regional Dissection for Molecular Analysis

DOI :

10.3791/61922-v

December 5th, 2020

December 5th, 2020

3,600 Views

1Department of Neuroscience, University of Minnesota

Different cerebellar regions have been implicated to play a role in distinct behavioral outputs, yet the underlying molecular mechanisms remain unknown. This work describes a method to reproducibly and quickly dissect cerebellar cortex of the hemispheres, anterior and posterior regions of the vermis, and the deep cerebellar nuclei in order to probe for molecular differences by isolating RNA and testing for differences in gene expression. 

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