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Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

DOI :

10.3791/58955-v

March 5th, 2019

March 5th, 2019

13,390 Views

1Oncology Research, Medimmune, LLC, 2Division of Rheumatology, Johns Hopkins University School of Medicine

Here, we present a flow cytometric protocol to identify CD4+ and CD8+ T cells, γδ T cells, B cells, NK cells and monocytes in human peripheral blood by using only two fluorochromes instead of seven. With this approach, five additional markers can be recorded on most flow cytometers.

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Keywords Flow Cytometry

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