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Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

DOI :

10.3791/59073-v

6:24 min

February 13th, 2019

February 13th, 2019

7,625 Views

1Cell Biology Center, Institute of Innovative Research, Tokyo Institute of Technology

Fluorescence resonance energy transfer-based real-time observation systems of the DNA strand exchange reaction mediated by Rad51 were developed. Using the protocols presented here, we are able to detect the formation of reaction intermediates and their conversion into products, while also analyzing the enzymatic kinetics of the reaction.

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Keywords DNA Strand Exchange

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