JoVE Logo
Faculty Resource Center

Sign In

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

DOI :

10.3791/59192-v

February 11th, 2019

February 11th, 2019

7,588 Views

1Department of Biology, Boston University, 2Bioinformatics Program, Boston University

Here, we present an enhanced yeast one-hybrid screening protocol to identify the transcription factors (TFs) that can bind to a human DNA region of interest. This method uses a high-throughput screening pipeline that can interrogate the binding of >1,000 TFs in a single experiment.

-- Views

Related Videos

article

Methyl-binding DNA capture Sequencing for Patient Tissues

article

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions

article

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

article

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens

article

Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells

article

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

article

Expression, Purification, and Liposome Binding of Budding Yeast SNX-BAR Heterodimers

article

Identification of Host Pathways Targeted by Bacterial Effector Proteins using Yeast Toxicity and Suppressor Screens

article

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

article

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved