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Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment

DOI :

10.3791/59195-v

April 22nd, 2019

April 22nd, 2019

11,126 Views

1Department of Radiation Oncology, University of Colorado Denver - Anschutz Medical Campus, 2Department of Anesthesiology, University of Colorado Denver - Anschutz Medical Campus, 3Division of Radiology, University of Colorado Denver - Anschutz Medical Campus

Here we present a reproducible method for developing an orthotopic murine model of head and neck squamous cell carcinoma. Tumors demonstrate clinically relevant histopathological features of the disease, including necrosis, poor differentiation, nodal metastases, and immune infiltration. Tumor-bearing mice develop clinically relevant symptoms including dysphagia, jaw displacement, and weight loss.

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Intramucosal Inoculation

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