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Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model

DOI :

10.3791/52657-v

April 13th, 2015

April 13th, 2015

19,704 Views

1Division of Oncology, Department of Medicine, Washington University School of Medicine, 2Palo Alto Institute for Research and Education, Veterans Affairs Palo Alto Health Care System, 3Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University School of Medicine

This protocol describes a method for the detailed evaluation of leukocyte subsets within the tumor microenvironment in a mouse tumor model. Chemerin-expressing B16 melanoma cells were implanted subcutaneously into syngeneic mice. Cells from the tumor microenvironment were then stained and analyzed by flow cytometry, allowing for detailed leukocyte subset analyses.

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Tumor infiltrating Leukocyte Subsets

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