September 25th, 2010
•Using fine tip micropipettes we inject plasmid DNA into subdomains of chicken somites or neural tubes. The concentration of the plasmid is adjusted to generate single transfected cells. We then allow the cells to develop into clonal populations.
Tags
Related Videos
Dissection of 6.5 dpc Mouse Embryos
In Ovo Electroporations of HH Stage 10 Chicken Embryos
Method for Culture of Early Chick Embryos ex vivo (New Culture)
Double Whole Mount in situ Hybridization of Early Chick Embryos
Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube
Single-cell Suction Recordings from Mouse Cone Photoreceptors
Two-Photon-Based Photoactivation in Live Zebrafish Embryos
Imaging Cell Shape Change in Living Drosophila Embryos
Single Cell Fate Mapping in Zebrafish
Facial Transplants in Xenopus laevis Embryos
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved