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Longitudinal Intravital Imaging of Brain Tumor Cell Behavior in Response to an Invasive Surgical Biopsy

DOI :

10.3791/59278-v

9:17 min

May 3rd, 2019

May 3rd, 2019

6,907 Views

1Cancer Genomics Netherlands, Prinses Máxima Center for Pediatric Oncology

Here we describe a method for high-resolution time-lapse multiphoton imaging of brain tumor cells before and after invasive surgical intervention (e.g., biopsy) within the same living animal. This method allows studying the impact of these invasive surgical procedures on tumor cells' migratory, invasive, and proliferative behavior at a single cell level.

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Longitudinal Intravital Imaging

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