Reverse Crosslinking, Purification of DNA, and Analysis of Digested Chromatin
6:33
Chromatin Immunoprecipitation
9:24
Results: Representative Microphotographs, Agarose Gel Analysis, and Occupancy in the AR Genome
10:42
Conclusion
필기록
In this protocol, we can identify the interactions between proteins and DNA, revealing the transcriptional regulation of the gene. This helps to understand the molecular mechanisms in the cells. We modify the pre-existing protocols and then establ
이 콘텐츠에 액세스하려면 로그인하거나 무료 평가판을 시작하세요
Chromatin immunoprecipitation (ChIP) is a powerful tool for understanding the molecular mechanisms of gene regulation. However, the method involves difficulties in obtaining reproducible chromatin fragmentation by mechanical shearing. Here, we provide an improved protocol for a ChIP assay using enzymatic digestion.