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Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

10.0K Views

09:37 min

May 2nd, 2019

DOI :

10.3791/59483-v

May 2nd, 2019


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Non reducing SDS PAGE

Chapters in this video

0:04

Title

0:34

Harvesting Cells After Blocking Free Cysteine Residues Using Iodoacetamide

2:02

Extraction of Protein and Sample Preparation

3:13

In Vivo Formaldehyde Cross-linking of Endogenous Proteins in U-2 OS Cells

4:30

Fractionation of Nuclei

6:11

Extraction of Protein and Sample Preparation

6:51

SDS-PAGE Analysis

7:53

Results: Analyzing Multimeric Complexes Using a Two-step Method of Non-reducing SDS-PAGE Analysis and Formaldehyde Cross-linking

9:04

Conclusion

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