JoVE Logo
Faculty Resource Center

Sign In

Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation

DOI :

10.3791/59664-v

June 7th, 2019

June 7th, 2019

6,977 Views

1Department of Biological Sciences, University of Southern California, Los Angeles, 2Department of Radiology and Developmental Neuroscience Program, Saban Research Institute, Children's Hospital Los Angeles, 3Keck School of Medicine, Department of Radiology, University of Southern California, Los Angeles

We report messenger RNA (mRNA) electroporation as a method that permits fast and efficient expression of multiple proteins in the quail embryo model system. This method can be used to fluorescently label cells and record their in vivo movements by time-lapse microscopy shortly after electroporation.

Tags

MRNA Electroporation

-- Views

Related Videos

article

Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach

article

Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes

article

Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments

article

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

article

Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

article

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

article

Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System

article

In Ovo Intravascular Injection in Chicken Embryos

article

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos

article

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved