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Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment

DOI :

10.3791/59677-v

May 14th, 2019

May 14th, 2019

8,344 Views

1Cancer Metastasis Laboratory, Department of Biomedicine, University of Basel and University Hospital Basel, 2SIB Swiss Institute of Bioinformatics

Here, we present an integrated workflow to identify phenotypic and molecular features that characterize circulating tumor cells (CTCs). We combine live immunostaining and robotic micromanipulation of single and clustered CTCs with single cell-based techniques for downstream analysis and assessment of metastasis-seeding ability.

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Micromanipulation

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