May 14th, 2019
•Here, we present an integrated workflow to identify phenotypic and molecular features that characterize circulating tumor cells (CTCs). We combine live immunostaining and robotic micromanipulation of single and clustered CTCs with single cell-based techniques for downstream analysis and assessment of metastasis-seeding ability.
Related Videos
Capture and Release of Viable Circulating Tumor Cells from Blood Video (Video) | JoVE
A Syngeneic Mouse Model of Metastatic Renal Cell Carcinoma for Quantitative and Longitudinal Assessment of Preclinical Therapies Video (Video) | JoVE
Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer Video (Video) | JoVE
Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization Video (Video) | JoVE
The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues Video (Video) | JoVE
Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH Video (Video) | JoVE
Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer Video (Video) | JoVE
Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids Video (Video) | JoVE
Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence Video (Video) | JoVE
Circulating Tumor Cell Lines: an Innovative Tool for Fundamental and Translational Research Video (Video) | JoVE
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved