Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins
24.0K Views
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10:21 min
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June 20th, 2019
DOI :
June 20th, 2019
•Alexander K. H. Weiss1,2, Max Holzknecht1,2, Elia Cappuccio1,2, Ilaria Dorigatti1, Karin Kreidl1, Andreas Naschberger3, Bernhard Rupp3, Hubert Gstach4, Pidder Jansen-Dürr1,2
1Research Institute for Biomedical Aging Research, University of Innsbruck Austria, 2Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck Austria, 3Division of Genetic Epidemiology, Medical University of Innsbruck Austria, 4Faculty of Chemistry, Department of Organic Chemistry, University of Vienna Austria
Chapters in this video
0:04
Title
1:12
Expression of FAHD Proteins in Competent E. coli
3:44
Lysis of Bacterial Pellets and Filtration of Debris
4:33
Purification of His-tagged FAHD Proteins using Ni-NTA Affinity Chromatography
5:59
Purification of FAHD Proteins via Ion Exchange Chromatography
6:35
Basic FAHD Activity Assays with Substrates Oxaloacetate and Acetylpyruvate
7:53
Results: Expression and Purification of Fumarylacetoacetate Hydrolase Domain-containing Proteins
9:19
Conclusion
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