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Pyrosequencing for Microbial Identification and Characterization

DOI :

10.3791/50405-v

12:37 min

August 22nd, 2013

August 22nd, 2013

45,504 Views

1Center for Biotechnology Education, Krieger School of Arts and Sciences, Johns Hopkins University, 2Qiagen Sciences, Inc.

Pyrosequencing is a versatile technique that facilitates microbial genome sequencing that can be used to identify bacterial species, discriminate bacterial strains, and detect genetic mutations that confer resistance to anti-microbial agents. In this video, the procedure for microbial amplicon generation, amplicon pyrosequencing, and DNA sequence analysis will be demonstrated.

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Pyrosequencing

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