Pyrosequencing for Microbial Identification and Characterization

46.9K Views

12:37 min

August 22nd, 2013

DOI :

10.3791/50405-v

August 22nd, 2013


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Pyrosequencing

Chapters in this video

0:05

Title

2:40

Amplification of the Template

4:01

Vacuum Workstation Protocol

4:05

Immobilizing the Biotinylated PCR Products

5:06

Denaturation of DNA and Addition of Sequencing Primer

7:30

Annealing Sequencing Primer to DNA Strands

7:55

Pyromark Q24 Operation for Pyrosequencing

7:58

Preparing for a Run

8:58

Starting a Run

9:46

Completing a Run

10:03

Results: Pyrosequencing Identifies Bacteria and Detects Antibiotic Drug Resistance

11:35

Conclusion

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