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Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation

DOI :

10.3791/59999-v

July 6th, 2019

July 6th, 2019

23,078 Views

1Department of Anesthesiology and Intensive Care Medicine, University Hospital Bonn

Presented here is a step-by-step procedure to induce acute lung injury in mice by direct intratracheal lipopolysaccharide instillation and to perform FACS analysis of blood samples, bronchoalveolar lavage fluid, and lung tissue. Minimal invasiveness, simple handling, good reproducibility, and titration of disease severity are advantages of this approach.

Tags

Acute Lung Injury

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