Blood Sampling, Bronchoalveolar Lavage, Organ Harvesting
5:58
Tissue Preparation for FACS Analysis
8:57
Results: Validation of Murine Acute Lung Injury Model Against Control Animals
10:31
Conclusion
필기록
Reliable animal models are crucial components of basic research. Our murine approach allows answering immunological questions on the mechanistics and kinetics of acute lung injury development in mammals. Minimal invasiveness, simple handling, as w
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Presented here is a step-by-step procedure to induce acute lung injury in mice by direct intratracheal lipopolysaccharide instillation and to perform FACS analysis of blood samples, bronchoalveolar lavage fluid, and lung tissue. Minimal invasiveness, simple handling, good reproducibility, and titration of disease severity are advantages of this approach.