Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

9.3K Views

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11:27 min

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September 18th, 2019

DOI :

10.3791/60045-v

September 18th, 2019

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Mohamed A. Sobhy*¹, Amer Bralić*¹, Vlad-Stefan Raducanu¹, Muhammad Tehseen¹, Yujing Ouyang¹, Masateru Takahashi¹, Fahad Rashid¹, Manal S. Zaher¹, Samir M. Hamdan¹

¹Laboratory of DNA Replication and Recombination, Biological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST)

Summary

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Single Molecule F rster Resonance Energy Transfer

Holliday Junction Resolution

Flap Endonuclease 1

Biomolecular Interactions

Single Molecule Methods

Optical Measurements

Flow Cell Preparation

Chapters in this video

0:04

Title

1:22

Flow Cell Preparation

3:01

Single-Molecule Förster Resonance Energy Transfer (smFRET) Experiments

6:25

Sm-FRET Data Analysis

7:10

Two-Color Alternating Excitation (ALEX) FRET Experiment and Analysis

8:24

Results: Representative Real-Time HJ Resolution Investigation

10:13

Conclusion

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