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Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox

DOI :

10.3791/62378-v

7:12 min

July 5th, 2021

July 5th, 2021

2,756 Views

1Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield

This article provides step-by-step instructions for making fully-corrected accurate FRET measurements on individual, freely diffusing biomolecules using the open-source, inexpensive smfBox, from switch on, through alignment and focusing, to data collection and analysis.

Tags

Single molecule FRET

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