Preparation of Bacteria for In Vivo Infection Studies
3:09
Intra-cisternal Injection of Mice
5:07
Evaluation of Colony Forming Units (CFU) Counts in Peripheral Organs
6:39
Preparation of Brain Tissues for CFU Count
8:05
Results: Survival of Mice Following Intracisternal Injection of Wild-type and Mutant N. meningitidis
9:28
Conclusion
Transcript
Neisseria meningitidis is one of the most common causes of sepsis and meningitidis among the human population all over the world, and the bacteria is restricted to the human host. In this experimental protocol, we will describe the tailored proced
Sign in or start your free trial to access this content
Here, we describe a method to induce meningococcal meningitis through an intracisternal route of infection in adult mice. We present a step by step protocol of meningococcal infection from the preparation of inoculum to the intracisternal infection; then record the animal survival and evaluate the bacterial loads in murine tissues.