Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
7.5K Views
•
09:16 min
•
September 1st, 2019
DOI :
September 1st, 2019
•Chapters in this video
0:04
Title
1:16
crRNA Design and Preparation of Cas9/gRNA RNP Complexes
2:16
Electroporation of RNP Complexes into OCI-AML3 Cells
3:12
Screening and Selection of Cell Clones with Biallelic Deletions
5:51
Functional Analyses of Silencer Deletions with Real-time Quantitative RT-PCR
7:18
Results: RUNX1 Intronic Silencer Deletion
8:16
Conclusion
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