JoVE Logo
Faculty Resource Center

Sign In

Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays

DOI :

10.3791/58710-v

January 7th, 2019

January 7th, 2019

9,222 Views

1Tumor Initiation and Maintenance Program, Sanford Burnham Prebys Medical Discovery Institute, 2Blood Research Institute, Blood Center of Wisconsin, 3Department of Bioengineering, University of California, San Diego

This manuscript describes a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) CRISPR-Cas9-based method for simple and expeditious investigation of the role of multiple candidate genes in Acute Myeloid Leukemia (AML) cell proliferation in parallel. This technique is scalable and can be applied in other cancer cell lines as well.

Tags

Keywords CRISPR Cas9

-- Views

Related Videos

article

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion

article

Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies

article

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics

article

Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion

article

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

article

Assessment of the Metabolic Profile of Primary Leukemia Cells

article

Yeast As a Chassis for Developing Functional Assays to Study Human P53

article

Patient-derived Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae as Hosts for Comparative Drug Assessment

article

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

article

Assays for Validating Histone Acetyltransferase Inhibitors

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved