Abbiategrasso Brain Bank Protocol for Collecting, Processing and Characterizing Aging Brains

17.1K Views

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12:28 min

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June 3rd, 2020

DOI :

10.3791/60296-v

June 3rd, 2020

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Tino Emanuele Poloni*¹^,⁴, Valentina Medici*¹, Arenn Faye Carlos¹, Annalisa Davin², Arcangelo Ceretti¹, Michela Mangieri¹, Paola Cassini¹^,⁴, Roberta Vaccaro³, Daniele Zaccaria³, Simona Abbondanza³, Matteo Bordoni⁵, Valentina Fantini²^,⁶, Elena Fogato⁷, Cristina Cereda⁵, Mauro Ceroni⁸, Antonio Guaita¹^,²^,³

¹Department of Neurology and Neuropathology, Golgi-Cenci Foundation, ²Laboratory of Neurobiology and Neurogenetic, Golgi-Cenci Foundation, ³Department of Neuropsychology and Social Sciences, Golgi-Cenci Foundation, ⁴Department of Rehabilitation, ASP Golgi-Redaelli Geriatric Hospital, ⁵Genomic and Post-Genomic Center, IRCCS Mondino Foundation, ⁶Department of Brain and Behavioral Sciences, University of Pavia, ⁷Department of Pathology, ASP Golgi-Redaelli Geriatric Hospital, ⁸Department of Neurological Science, IRCCS Mondino Foundation, University of Pavia

Summary

Chapters in this video

0:04

Introduction

1:28

Time of Death and Brain Removal

5:23

Abbiategrasso Brain Bank (ABB) Dissection

8:26

Tissue Freezing and Fixation

9:17

Results: Representative Brain Specimen Characterization and Analyses

11:13

Conclusion

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