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Isolation of Cerebral Capillaries from Fresh Human Brain Tissue

DOI :

10.3791/57346-v

September 12th, 2018

September 12th, 2018

12,202 Views

1Sanders-Brown Center on Aging, Department of Pharmacology and Nutritional Sciences, University of Kentucky, 2Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 3Department of Neuroscience, University of Kentucky

Isolated brain capillaries from human brain tissue can be used as a preclinical model to study barrier function under physiological and pathophysiological conditions. Here, we present an optimized protocol to isolate brain capillaries from fresh human brain tissue.

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