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Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform

DOI :

10.3791/60383-v

October 6th, 2019

October 6th, 2019

6,337 Views

1Department of Biomedical Engineering, University of Delaware, 2Department of Materials Science & Engineering, University of Delaware, 3Delaware Biotechnology Institute

This protocol provides instructions for implementing multiphoton lithography to fabricate three-dimensional arrays of fluorescent fiducial markers embedded in poly(ethylene glycol)-based hydrogels for use as reference-free, traction force microscopy platforms. Using these instructions, measurement of 3D material strain and calculation of cellular tractions is simplified to promote high-throughput traction force measurements.

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Traction Force Microscopy

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