Transfection, Chemical Fixation and Peroxidase Reaction
3:48
High Pressure Freezing
4:46
Freeze Substitution
6:23
Resin Infiltration and Embedding
8:11
Sectioning and TEM Imaging
9:17
Results: TEM Images
11:07
Conclusion
Transcript
This method combines the ability to stain for a membrane protein while also preserving membrane integrity and subcellular architecture. So it's really powerful for precisely localizing a membrane associated protein. The main advantage of this tech
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This protocol describes the cryoAPEX method, in which an APEX2-tagged membrane protein can be localized by transmission electron microscopy within optimally-preserved cell ultrastructure.