We present a protocol for the electron microscopic visualization of the replicative domains in the cell by the EdU labeling of the newly synthesized DNA and label detection with silver enhancement of Nanogold particles and ChromEM staining of the
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This protocol presents a technique for high-resolution mapping of replication sites in structurally preserved chromatin in situ that employs a combination of pre-embedding EdU-streptavidin-Nanogold labeling and ChromEMT.