Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
3.6K Views
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14:56 min
•
May 20th, 2022
DOI :
May 20th, 2022
•Sophie Sosnovskaya*1,2, Amir N. Zakirov*1,2, Ekaterina D. Ryumina*1,3, Ekaterina Kharybina1,2, Sergei A. Golyshev1, Olga S. Strelkova1, Oxana A. Zhironkina1, Andrei Moiseenko2, Anton Orekhov4, Igor I. Kireev1,2,5
1Belozersky Institute of Physico-chemical Biology, Lomonosov Moscow State University, 2Faculty of Biology, Lomonosov Moscow State University, 3Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 4National Research Center, Kurchatov Institute, 5V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology
Chapters in this video
0:08
Introduction
0:35
Cells Labeling and Fixation
2:13
Click-Reaction
5:09
Ag-Amplification
6:28
Gold Toning
6:42
ChromEM
7:23
Dehydration and Epon Embedding
12:50
Electron Tomography
13:06
Representative Results
14:14
Conclusions
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