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Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy

DOI :

10.3791/60784-v

January 25th, 2020

January 25th, 2020

5,604 Views

1Department of Bioengineering, Lehigh University, 2Department of Materials Science and Engineering, Lehigh University, 3Department of Chemistry, Lehigh University, 4Department of Mechanical Engineering and Mechanics, Lehigh University

We present a protocol for immobilizing single macromolecules in microfluidic devices and quantifying changes in their conformations under shear flow. This protocol is useful for characterizing the biomechanical and functional properties of biomolecules such as proteins and DNA in a flow environment.

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Single molecule Conformational Changes

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