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Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection

DOI :

10.3791/50969-v

February 18th, 2014

February 18th, 2014

8,822 Views

1Department of Chemistry and Biochemistry, Florida International University

Here we report an application of the photothermal beam deflection technique in combination with a caged calcium compound, DM-nitrophen, to monitor microsecond and millisecond dynamics and energetics of structural changes associated with the calcium association to a neuronal calcium sensor, Downstream Regulatory Element Antagonist Modulator.

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