Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions
14.6K Views
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10:24 min
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May 24th, 2020
DOI :
May 24th, 2020
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Chapters in this video
0:04
Introduction
0:50
Human Pluripotent Stem Cell (hPSC) Maintenance
1:48
hPSC Differentiation (Day 0)
3:34
Neural Crest Cell (NCC) Induction (Day 1-10)
4:02
NC Spheroid Aggregation (Day 10)
5:14
NC Spheroid Maintenance and Sympathetic Progenitor Induction (Day 11-14)
6:09
Sympathetic Neuron (symN) Differentiation and Maturation (Day 14-20 or 28-35)
7:38
Results: Representative NC Induction, Maintenance, and Expansion
9:43
Conclusion
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