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TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

DOI :

10.3791/60970-v

7:44 min

June 8th, 2020

11,364 Views

1Department of Pediatrics, University of Florida

We present an optimized tandem mass tag (TMT) labeling protocol that includes detailed information for each of the following steps: protein extraction, quantification, precipitation, digestion, labeling, submission to a proteomics facility, and data analyses.

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