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TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

DOI :

10.3791/60970-v

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7:44 min

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June 8th, 2020

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11,364 Views

1Department of Pediatrics, University of Florida

We present an optimized tandem mass tag (TMT) labeling protocol that includes detailed information for each of the following steps: protein extraction, quantification, precipitation, digestion, labeling, submission to a proteomics facility, and data analyses.

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