JoVE Logo
Faculty Resource Center

Sign In

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

DOI :

10.3791/60970-v

7:44 min

June 8th, 2020

June 8th, 2020

11,864 Views

1Department of Pediatrics, University of Florida

We present an optimized tandem mass tag (TMT) labeling protocol that includes detailed information for each of the following steps: protein extraction, quantification, precipitation, digestion, labeling, submission to a proteomics facility, and data analyses.

Tags

TMT Sample Preparation

-- Views

Related Videos

article

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

article

Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows

article

Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics

article

Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules

article

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

article

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

article

A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline

article

Preparation of High-Temperature Sample Grids for Cryo-EM

article

Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot

article

Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved