Off‐Line HpH‐RP Chromatographic Fractionation of Peptides
2:53
R‐Methylated Peptide Immuno‐Affinity Enrichment
6:47
Results: R‐Methylation Analysis by Mass Spectrometry
8:39
Conclusion
Transcript
This protocol is highly significant because it represents the state-of-the-art workflow for the global analysis of protein arginine methylation by mass spectrometry. This is the only method to identify and profile R-methylated protein with a singl
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Protein Arginine (R)-methylation is a wide-spread post-translational modification regulating multiple biological pathways. Mass spectrometry is the best technology to globally profile the R-methyl-proteome, when coupled to biochemical approaches for modified peptide enrichment. The workflow designed for the high confidence identification of global R-methylation in human cells is described here.