In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time

8.7K Views

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09:19 min

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May 24th, 2020

DOI :

10.3791/61068-v

May 24th, 2020

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Xue D. Manz¹, Hugo J. Albers²^,³, Petr Symersky⁴, Jurjan Aman¹, Andries D. van der Meer³, Harm Jan Bogaard¹, Robert Szulcek¹^,⁵^,⁶

¹Department of Pulmonary Diseases, Amsterdam UMC, VU University Medical Center, Amsterdam Cardiovascular Sciences (ACS), ²BIOS Lab-on-a-Chip group, University of Twente, ³Applied Stem Cell Technologies Group, University of Twente, ⁴Department of Cardio-thoracic Surgery, Amsterdam UMC, VU University Medical Center, ⁵Institute of Physiology, Charité-Universitätsmedizin, ⁶German Heart Center

Summary

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In Vitro Disease Model

Microfluidics

Endothelial Interactions

Personalized Medicine

Cell Culture

Fibronectin

Endothelial Cell Medium

Flow Cytometry

Magnetic Affinity Cell Separation

Primary Endothelial Cells

Flow Chamber Preparation

Chapters in this video

0:04

Introduction

0:59

Primary Human Pulmonary Arterial Endothelial Cell (PAEC) Isolation and Culture

2:43

Flow Chambers and PAEC Monolayer Preparation

4:00

Washed Human Whole Blood Preparation

4:50

Flow System Assembly

5:41

Washed Whole Blood Perfusion

6:44

Results: Representative Thrombus Formation Characterization

8:29

Conclusion

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