Name: using q suture to enhance resistance to gap formation and tensile strength of repaired flexor tendons
Uploaded: 2020-06-03T15:00:00
Description: Watch this Scientific Journal Video about Using Q Suture to Enhance Resistance to Gap Formation and Tensile Strength of Repaired Flexor Tendons at JoVE.com

The authors acknowledge support from Graduate Research Innovation Project of Jiangsu Province (YKC16061).

All experimental procedures described were approved by the Administration Committee of Experimental Animals of the Nantong University. Thirty porcine tendons were repaired with three 2-strand repairs: 2-strand core suture, 2-strand core suture plus 2Q, and 2-strand core suture plus running peripheral sutures. The other 30 porcine tendons were repaired with three 4 strand repairs: 4-strand core suture, 4-strand core suture plus 2Q, and 4-strand core suture plus running peripheral sutures.
1. Prep...

<ol>
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The results of the current study showed that Q suture not only reduced the gapping and improves tensile strength of the repaired tendons but was also timesaving and labor-saving. Nonetheless, some key points regarding tendon repair in the current study should be noted.
First, we tried to select tendon samples that were similar in shape and size because we were not sure whether tendon size would have a notable impact on tensile strength after repair. In addition, tendon samples can be preserved...

Gap formation at tendon repair site affects tendon repair strength and gliding resistance substantially. The consequences of gapping between tendon ends may ultimately impede tendon healing in vivo1. It has been reported that the presence of a gap larger than 2 mm at the repair site lead to a significant increase in the gliding resistance of repaired intrasynovial tendon in cadaveric hands2. A study in a canine model has shown that a gap size larger than 3 mm would impair the tendon healing strength and stiffness3. Therefore, improving resistance and decreasing the risk of gapping between tendon ends are critical for tendon repair.
Addition of peripheral sutures has been shown to reduce the gapping at the tendon repair site thereby improving gliding function of the repaired tendons4,5,6. During the last few decades, a number of peripheral sutures have been developed, including the interlocking cross stitch (IXS), interlocking horizontal mattress (IHM), and cross-linked Silfverski&#246;ld and Lembert, et al7,8,9,10. These peripheral sutures have proven to be superior to running peripheral sutures with respect to gapping resistance in tendon repair. However, many of these sutures are complex in structure and difficult to perform, thereby limiting their widespread applications. An ideal suture for tendon repair should aim to prevent gap formation while avoiding the addition of bulk to the repair site after tendon repair. Currently, running peripheral suture remains a popular technique due to its simplicity.
In a recent study, a technique, alternative to peripheral suture, named Q suture, because its shape is similar to the letter &#8220;Q&#8221;, is presented11. Here, we compared this suturing technique with running peripheral suture to check for the differences in gapping resistance and the tensile strength of repaired tendons. The results showed that Q suture was more efficient in enhancing the gapping resistance and ultimate strength of the repaired tendons in the cyclic loading test. Therefore, this article aims to provide a detailed description of how to perform Q suture technique and the biomechanical settings for testing the effects of Q suture on the properties of the repaired tendons.

<table>
	<tbody>
		<tr>
			<td>4-0 suture</td>
			<td>Ethicon, Somerville, NJ</td>
			<td>Ethilon 1667</td>
			<td></td>
		</tr>
		<tr>
			<td>6-0 suture</td>
			<td>Ethicon, Somerville, NJ</td>
			<td>Ethilon 689</td>
			<td></td>
		</tr>
		<tr>
			<td>biomechanical testing machine</td>
			<td>Instron Corp, Norwood, MA</td>
			<td>Instron 3365</td>
			<td></td>
		</tr>
		<tr>
			<td>biomechanical testing software</td>
			<td>Instron Corp, Norwood, MA</td>
			<td>Bluehill 2</td>
			<td></td>
		</tr>
	</tbody>
</table>

using q suture to enhance resistance to gap formation and tensile strength of repaired flexor tendons

Peripheral epitendinous sutures are believed to enhance core suture strength in tendon repair and decrease the risk of gapping between tendon ends. Here Q suture, an alternative to peripheral sutures, is presented for the use in tendon repair. Its effects on gap formation and tensile strength of the repaired tendons were compared with conventional running peripheral sutures. Three 2-strand sutures and three 4-strand sutures were used in repairing porcine tendons. The time required for performing 2Q and running sutures were recorded. The repaired tendons were subjected to a cyclic loading test, and the cycle number, during which a 2-mm gap was formed, was determined. After the cyclic loading, the gap size at the tendon ends and the ultimate strength of the repaired tendons were measured. Augmentation with the Q sutures reduced the number of tendons showing 2-mm gaps at tendon ends during cyclic loading. With addition of Q sutures 2-strand sutures significantly increased the ultimate strength of the repaired tendons and 4-strand sutures decreased the gap distance at the repair site of tendons. The time required for performing 2Q sutures was significantly less than that for running sutures. Therefore, we conclude that the Q suture is efficient in enhancing the tensile resistance and tendon repair strength and can be an alternative to conventional peripheral sutures.

Table 1 shows that addition of Q suture reduced the number of tendons with 2-mm gapping during cyclic loading in both 2-strand and 4-strand repairs. All tendons repaired with 2-strand and 4-strand core sutures formed a 2-mm gap, whereas none of the tendons repaired with 2-strand plus 2Q and only half of those repaired with 4-strand plus 2Q had a 2-mm gapping after 10 cycles. More tendons repaired with 2-strand plus running or 4-strand plus running sutures showed a 2-mm gap than those augmented with Q sut...

Watch this Scientific Journal Video about Using Q Suture to Enhance Resistance to Gap Formation and Tensile Strength of Repaired Flexor Tendons at JoVE.com

Using Q Suture to Enhance Resistance to Gap Formation and Tensile Strength of Repaired Flexor Tendons

Here, we present a &#8220;Q&#8221; suture technique that can be performed in tendon repair and its effects on the gap formation and tensile strength of the repaired tendons. Q suture is shown to be efficient in enhancing the tensile resistance and tendon repair strength.

Peripheral epitendinous sutures are believed to enhance core suture strength in tendon repair and decrease the risk of gapping between tendon ends. Here Q ...

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