Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
2.7K Views
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09:58 min
•
June 27th, 2020
DOI :
June 27th, 2020
•Iftekhar A. Showpnil1,2, Kyle R. Miller1, Cenny Taslim1, Kathleen I. Pishas1, Stephen L. Lessnick1,3, Emily R. Theisen1,4
1Center for Childhood Cancer and Blood Diseases, Abigail Wexner Research Institute at Nationwide Children's Hospital, 2Molecular, Cellular, and Developmental Biology Program, The Ohio State University, 3Division of Pediatric Hematology/Oncology/Blood & Marrow Transplant, The Ohio State University, 4Department of Pediatrics, The Ohio State University
Chapters in this video
0:04
Introduction
0:55
In Vitro Construct Panel Setup
2:07
Cell Collection
2:47
Construct Expression Validation and Correlative Phenotypic Assay Setup
3:50
Alignment and Transcript Counting Pipeline
5:32
Differential Expression
7:40
Results: Representative Disordered Oncogenic Transcription Factor Structure-Function Relationship Mapping
9:31
Conclusion
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