Cell Seeding for Transfection of Dual-Luciferase Reporter
6:27
Dual-Luciferase Reporter Transfection
7:35
Dual-Luciferase Activity Quantification
9:02
Results: Representative Arrayed Library Screening
10:47
Conclusion
Transcript
This protocol can be used to screen arrayed lentiviral or retroviral assay libraries to identify novel regulators of transcription factors in cancer cells. The main advantages of this technique are that it is rapid, medium throughput, and inexpens
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To identify novel regulators of transcription factors, we developed an approach to screen arrayed lentiviral or retroviral RNAi libraries using a dual-luciferase-based transcriptional reporter assay. This approach offers a quick and relatively inexpensive way to screen hundreds of candidates in a single experiment.