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Enabling Real-Time Compensation in Fast Photochemical Oxidations of Proteins for the Determination of Protein Topography Changes

DOI :

10.3791/61580-v

September 1st, 2020

September 1st, 2020

5,098 Views

1Department of Biomolecular Sciences, University of Mississippi, 2Department of Chemistry and Biochemistry, University of Mississippi, 3GenNext Technologies, Inc.

Fast photochemical oxidation of proteins is an emerging technique for the structural characterization of proteins. Different solvent additives and ligands have varied hydroxyl radical scavenging properties. To compare the protein structure in different conditions, real-time compensation of hydroxyl radicals generated in the reaction is required to normalize reaction conditions.

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Keywords Real time Compensation

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