Fast Photochemical Oxidations of Proteins (FPOP) Optical Bench Preparation
2:43
FPOP Experiment
3:58
Compensation Performance
5:45
Results: Representative Adalimumab FPOP and Compensated Myoglobin FPOP in MES Buffer
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Conclusion
Transcrição
The method facilitates measurement of protein confirmation and protein interactions. The buffer composition, sample purity and protein concentration requirements are flexible allowing the method to address challenging structural problems. This tec
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Fast photochemical oxidation of proteins is an emerging technique for the structural characterization of proteins. Different solvent additives and ligands have varied hydroxyl radical scavenging properties. To compare the protein structure in different conditions, real-time compensation of hydroxyl radicals generated in the reaction is required to normalize reaction conditions.