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An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis

5.3K Views

06:10 min

August 6th, 2020

August 6th, 2020

5,317 Views

0:04

Introduction

0:47

Preparation and Experimental Steps

3:26

SWIFT-Choroid Computerized Quantification Method

4:25

Results: Free Fatty Acid Receptor (FFAR) 4 Suppression Exacerbate Choroidal Neovascularization

5:38

Conclusion

Transcript

This Choroid assay is highly reproducible and pertinent to Choroidal Angiogenesis research and Age related Macular Degeneration, AMD It can compliment in vivo studies of microvascular behavior. This assay can be used to screen compounds as potenti

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This protocol presents choroid sprouting assay, an ex vivo model of microvascular proliferation. This assay can be used to assess pathways involved in proliferating choroidal micro vessels and assess drug treatments using wild type and genetically modified mouse tissue.

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