January 16th, 2021
•This article presents a method to generate protein crystals derivatized with I3C (5-amino-2,4,6-triiodoisophthalic acid) using microseeding to generate new crystallization conditions in sparse matrix screens. The trays can be set up using liquid dispensing robots or by hand.
Tags
Related Videos
Assaying Protein Kinase Activity with Radiolabeled ATP
Microcrystallography of Protein Crystals and In Cellulo Diffraction
Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering
High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique
Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays
Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization
Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved