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Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening

DOI :

10.3791/61894-v

14:04 min

January 16th, 2021

January 16th, 2021

4,257 Views

1School of Biological Sciences, The University of Adelaide, 2Institute of Photonics and Advanced Sensing (IPAS), School of Biological Sciences, The University of Adelaide

This article presents a method to generate protein crystals derivatized with I3C (5-amino-2,4,6-triiodoisophthalic acid) using microseeding to generate new crystallization conditions in sparse matrix screens. The trays can be set up using liquid dispensing robots or by hand.

Tags

Derivation

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