January 16th, 2011
•The 3-D structure of a molecule provides a unique understanding of how the molecule functions. The principal method for structure determination at near-atomic resolution is X-ray crystallography. Here, we demonstrate the current methods for obtaining three-dimensional crystals of any given macromolecule that are suitable for structure determination by X-ray crystallography.
Tags
Related Videos
Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery
Assessing Two-dimensional Crystallization Trials of Small Membrane Proteins for Structural Biology Studies by Electron Crystallography
Crystallization of Membrane Proteins in Lipidic Mesophases
High-throughput Crystallization of Membrane Proteins Using the Lipidic Bicelle Method
3D Printing of Preclinical X-ray Computed Tomographic Data Sets
Improving the Success Rate of Protein Crystallization by Random Microseed Matrix Screening
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved